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goat anti rgma  (R&D Systems)


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    R&D Systems goat anti rgma
    Goat Anti Rgma, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rgma+antibody/Mouse+RGM-A+Antibody/pmc11840113-350-60-64
    Average 93 stars, based on 8 article reviews
    goat anti rgma - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Article Snippet: .. COS-7 cells were incubated with goat anti-RGMa antibody (AF2458; R&D systems) 1∶200, sheep anti-RGMb antibody (AF3597; R&D systems) 1∶50 or goat anti-Neogenin antibody (AF1079; R&D systems) 1∶50 in normal blocking buffer for 2 hrs at RT. .. Cells were washed in PBS and incubated with the appropriate Alexa Fluor-labeled secondary antibodies (Invitrogen) 1∶500 at RT.

    Article Title: Accumulation of a repulsive axonal guidance molecule RGMa in amyloid plaques: a possible hallmark of regenerative failure in Alzheimer's disease brains.
    Article Snippet: Aims: RGMa is a repulsive guidance molecule that induces the collapse of axonal growth cones by interacting with the receptor neogenin in the central nervous system during development.. It remains unknown whether RGMa plays a role in the neurodegenerative process of Alzheimer’s disease (AD).. We hypothesize that RGMa, if it is concentrated on amyloid plaques, might contribute to a regenerative failure of degenerating axons in AD brains.

    Article Title: Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Article Snippet: .. In brief, sections were washed in PBS (pH 7.4) and incubated in normal blocking buffer (PBS, 4% BSA and 0.1% Triton) for 1 hr at RT and incubated with goat anti-RGMa antibody (AF2458; R&D systems) 1∶200, sheep anti-RGMb antibody (AF3597; R&D systems) 1∶200 or goat anti-Neogenin antibody (AF1079; R&D systems) 1∶200 overnight in normal blocking buffer at 4°C. ..

    Blocking Assay:

    Article Title: Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Article Snippet: .. COS-7 cells were incubated with goat anti-RGMa antibody (AF2458; R&D systems) 1∶200, sheep anti-RGMb antibody (AF3597; R&D systems) 1∶50 or goat anti-Neogenin antibody (AF1079; R&D systems) 1∶50 in normal blocking buffer for 2 hrs at RT. .. Cells were washed in PBS and incubated with the appropriate Alexa Fluor-labeled secondary antibodies (Invitrogen) 1∶500 at RT.

    Article Title: Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Article Snippet: .. In brief, sections were washed in PBS (pH 7.4) and incubated in normal blocking buffer (PBS, 4% BSA and 0.1% Triton) for 1 hr at RT and incubated with goat anti-RGMa antibody (AF2458; R&D systems) 1∶200, sheep anti-RGMb antibody (AF3597; R&D systems) 1∶200 or goat anti-Neogenin antibody (AF1079; R&D systems) 1∶200 overnight in normal blocking buffer at 4°C. ..

    Recombinant:

    Article Title: Accumulation of a repulsive axonal guidance molecule RGMa in amyloid plaques: a possible hallmark of regenerative failure in Alzheimer's disease brains.
    Article Snippet: Aims: RGMa is a repulsive guidance molecule that induces the collapse of axonal growth cones by interacting with the receptor neogenin in the central nervous system during development.. It remains unknown whether RGMa plays a role in the neurodegenerative process of Alzheimer’s disease (AD).. We hypothesize that RGMa, if it is concentrated on amyloid plaques, might contribute to a regenerative failure of degenerating axons in AD brains.



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    goat anti rgma  (R&D Systems)
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    Goat Anti Rgma, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat polyclonal anti rgma  (R&D Systems)
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    goat anti mouse rgma antibody  (R&D Systems)
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    R&D Systems goat anti mouse rgma antibody
    AAV vectors direct <t>RGMa</t> expression and secretion in a neuronal cell line and dose-dependent expression in the mouse brain. A, AAV plasmid-mediated overexpression of mouse RGMa in N2A cells results in the production and secretion of RGMa protein. N2A cells were either untreated (UnTr) or transfected with the expression plasmids pAAV-SYN-GFP or pAAV-SYN-mRGMa. Anti-mouse RGMa antibody (R&D <t>Systems,</t> <t>AF2458)</t> was used to detect mouse RGMa protein in the cell lysate (LYSATE samples) or the culture medium (MEDIUM samples) 3 d after transfection. N2A cells produce mouse RGMa after transfection of the expected molecular weight: multiple bands are visible ∼49–55 kDa representing the full-length form, and one prominent band at 33 kDa representing the cleaved membrane bound C-terminal form. RGMa is also released from the transfected N2A cells, which results in the N-terminal 30 kDa form of RGMa in the medium samples. β-actin was used as loading control. B, Comparison of RGMa protein levels in the SN of mouse injected with a high titer (9.0 × 1012 gc/ml) and low titer (3.0 × 1012 gc.ml). The RGMa fluorescence was twice as high in the high-titer-injected mice compared with the low-titer-injected mice (p < 0.0001, F(3,22) = 41.21). Data are represented in percentages of RGMa fluorescence levels relative to the AAV-Empty RGMa. Tissue from 6 mice was quantified in the high-titer treatment group, and from 7–8 mice in low-titer treatment group. Example images used for the quantification of RGMa are shown in Figure 6B (high-dose experiment) and Figure 7B (low-dose experiment). Statistical analysis was performed using one-way ANOVA with Tukey's post hoc multiple testing correction: ***p < 0.001; ****p < 0.0001. All data are expressed as mean with bars representing SEM.
    Goat Anti Mouse Rgma Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rgma+antibody/Mouse+RGM-A+Antibody/pmc06596765-135-4-9
    Average 94 stars, based on 1 article reviews
    goat anti mouse rgma antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    AAV vectors direct RGMa expression and secretion in a neuronal cell line and dose-dependent expression in the mouse brain. A, AAV plasmid-mediated overexpression of mouse RGMa in N2A cells results in the production and secretion of RGMa protein. N2A cells were either untreated (UnTr) or transfected with the expression plasmids pAAV-SYN-GFP or pAAV-SYN-mRGMa. Anti-mouse RGMa antibody (R&D Systems, AF2458) was used to detect mouse RGMa protein in the cell lysate (LYSATE samples) or the culture medium (MEDIUM samples) 3 d after transfection. N2A cells produce mouse RGMa after transfection of the expected molecular weight: multiple bands are visible ∼49–55 kDa representing the full-length form, and one prominent band at 33 kDa representing the cleaved membrane bound C-terminal form. RGMa is also released from the transfected N2A cells, which results in the N-terminal 30 kDa form of RGMa in the medium samples. β-actin was used as loading control. B, Comparison of RGMa protein levels in the SN of mouse injected with a high titer (9.0 × 1012 gc/ml) and low titer (3.0 × 1012 gc.ml). The RGMa fluorescence was twice as high in the high-titer-injected mice compared with the low-titer-injected mice (p < 0.0001, F(3,22) = 41.21). Data are represented in percentages of RGMa fluorescence levels relative to the AAV-Empty RGMa. Tissue from 6 mice was quantified in the high-titer treatment group, and from 7–8 mice in low-titer treatment group. Example images used for the quantification of RGMa are shown in Figure 6B (high-dose experiment) and Figure 7B (low-dose experiment). Statistical analysis was performed using one-way ANOVA with Tukey's post hoc multiple testing correction: ***p < 0.001; ****p < 0.0001. All data are expressed as mean with bars representing SEM.

    Journal: The Journal of Neuroscience

    Article Title: Repulsive Guidance Molecule a (RGMa) Induces Neuropathological and Behavioral Changes That Closely Resemble Parkinson's Disease

    doi: 10.1523/JNEUROSCI.0084-17.2017

    Figure Lengend Snippet: AAV vectors direct RGMa expression and secretion in a neuronal cell line and dose-dependent expression in the mouse brain. A, AAV plasmid-mediated overexpression of mouse RGMa in N2A cells results in the production and secretion of RGMa protein. N2A cells were either untreated (UnTr) or transfected with the expression plasmids pAAV-SYN-GFP or pAAV-SYN-mRGMa. Anti-mouse RGMa antibody (R&D Systems, AF2458) was used to detect mouse RGMa protein in the cell lysate (LYSATE samples) or the culture medium (MEDIUM samples) 3 d after transfection. N2A cells produce mouse RGMa after transfection of the expected molecular weight: multiple bands are visible ∼49–55 kDa representing the full-length form, and one prominent band at 33 kDa representing the cleaved membrane bound C-terminal form. RGMa is also released from the transfected N2A cells, which results in the N-terminal 30 kDa form of RGMa in the medium samples. β-actin was used as loading control. B, Comparison of RGMa protein levels in the SN of mouse injected with a high titer (9.0 × 1012 gc/ml) and low titer (3.0 × 1012 gc.ml). The RGMa fluorescence was twice as high in the high-titer-injected mice compared with the low-titer-injected mice (p < 0.0001, F(3,22) = 41.21). Data are represented in percentages of RGMa fluorescence levels relative to the AAV-Empty RGMa. Tissue from 6 mice was quantified in the high-titer treatment group, and from 7–8 mice in low-titer treatment group. Example images used for the quantification of RGMa are shown in Figure 6B (high-dose experiment) and Figure 7B (low-dose experiment). Statistical analysis was performed using one-way ANOVA with Tukey's post hoc multiple testing correction: ***p < 0.001; ****p < 0.0001. All data are expressed as mean with bars representing SEM.

    Article Snippet: Blots were incubated with goat anti-mouse RGMa antibody (1:100, R&D Systems, AF2458, an antibody raised against amino acids 48–421 of human RGMa) and β-actin (1:1000, Sigma-Aldrich, A5316) at 4°C overnight in block mix.

    Techniques: Expressing, Plasmid Preparation, Over Expression, Transfection, Molecular Weight, Membrane, Control, Comparison, Injection, Fluorescence